Rabbit anti-Phospho-NFKB p65(Thr254) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C

Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Anti

Sample: Lung (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti- phospho-NFKB p65(Thr254) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 61 kD Observed band size: 61 kD

Overlay histogram showing HL 60 cells stained with abs155805 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-

Blank control:A431. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr254) antibody Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. P

Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr254) antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg