Rabbit anti-Phospho-HSP27(Ser15) Polyclonal Antibody

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-HSP27 (Ser15)) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; follo

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C f

Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-Phospho-HSP27 (Ser15) antibody ,Dilution: 1μg /10^6 cells

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2:U251 (Human) Cell Lysate at 30 ug Primary: Anti-Phospho-HSP27 (Ser15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band si