Rabbit anti-Phospho-EGFR(Tyr1068) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for

Blank control (blue line): A431 (blue). Primary Antibody (green line): Rabbit Anti- Phospho-EGFR (Tyr1068) antibody Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-ra

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-EGFR (Tyr1068)) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; foll