Rabbit anti-Osteopontin Polyclonal Antibody
折上赠货号-规格 | 货期 | 价格 | 数量 |
abs119627-50uL | 1-2周 | ¥1500.00 | - + |
abs119627-100uL | 1-2周 | ¥2300.00 | - + |
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Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-osteopontin (abs119627) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 50 kD | ||
Tissue/cell: rat shin bone; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated secondary primary antibody 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated secondary primary antibody 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
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