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  • Rabbit Anti-osteopontin Polyclonal Antibody

    • osteopontin抗体,osteopontin, BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, Secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin, OSTP_Mouse. , ,
    货号: abs119627
    产品说明书
    分享:
    货号-规格 货期 价格 数量
    abs119627-50ul 1-2周 ¥1500.00
    - +
    abs119627-100ul 1-2周 ¥2300.00
    - +
    大包装询价
    概述
    别名
    osteopontin抗体;osteopontin, BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, Secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin, OSTP_Mouse. , ,
    宿主
    Rabbit
    特异性
    osteopontin
    反应种属
    Mouse;Rat;
    应用
    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
    分子量
    Predicted molecular weight: 34kDa
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    免疫原
    KLH conjugated synthetic peptide derived from mouse OPN:221-280/294 
    性能
    浓度
    1mg/ml
    纯化方法
    affinity purified by Protein A
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    存储溶液
    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    研究领域
    神经生物学  信号转导  
    靶标
    背景说明
    Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
    细胞定位
    分泌型蛋白 
    UniProt
    Sample:
    Kidney (Mouse) Lysate at 40 ug
    Primary: Anti-osteopontin (abs119627) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 34 kD
    Observed band size: 50 kD
    Tissue/cell: rat shin bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
    Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated secondary primary antibody 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
    Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated secondary primary antibody 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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