Rabbit anti-Myoglobin Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for

Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myoglobin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myoglobin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to th

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myoglobin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjuga

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myoglobin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjuga

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myoglobin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to th

Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Primary: Anti-Myoglobin at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD