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Rabbit anti-KLF2 Polyclonal AntibodyRabbit anti-KLF2 Polyclonal AntibodyRabbit anti-KLF2 Polyclonal AntibodyRabbit anti-KLF2 Polyclonal Antibody
  • Rabbit anti-KLF2 Polyclonal Antibody
  • Rabbit anti-KLF2 Polyclonal Antibody
  • Rabbit anti-KLF2 Polyclonal Antibody
  • Rabbit anti-KLF2 Polyclonal Antibody
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  • Rabbit anti-KLF2 Polyclonal Antibody

    货号-规格 货期 价格 数量
    abs124444-50uL 1-2周 ¥1500.00
    - +
    abs124444-100uL 1-2周 ¥2300.00
    - +
    大包装询价
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    确定
    Sample:
    Lane1:Brain (Mouse) Lysate at 30 ug
    Lane2:HepG2 Cell Lysate at 30 ug
    Primary: Anti-KLF2 at 1:300 dilution;
    Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000 dilution;
    Predicted band size :39 kD
    Observed band size :39 kD
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining.
    Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer at 37°C for 20 min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
    Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer, Boiling bathing for 15min; Blocking buffer at 37℃ for 20 min;
    Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
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