Rabbit anti-KLF2 Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs124444-50uL | 1-2周 | ¥1500.00 | - + |
abs124444-100uL | 1-2周 | ¥2300.00 | - + |
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Sample: Lane1:Brain (Mouse) Lysate at 30 ug Lane2:HepG2 Cell Lysate at 30 ug Primary: Anti-KLF2 at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000 dilution; Predicted band size :39 kD Observed band size :39 kD | ||
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining. | ||
Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer at 37°C for 20 min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei. | ||
Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Blocking buffer at 37℃ for 20 min; Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei | ||
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