货号-规格 货期 价格 数量
abs132004-100uL 现货
1200 .00
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实验结果图
Sample: Lane 1: Cerebellum (Mouse) Lysate Lane 2: Raw264.7 (Mouse) Cell Lysate Lane 3: NIH/3T3 (Mouse) Cell Lysate Lane 4: Cerebrum (Rat) Lysate Lane 5: SH-SY5Y (Human) Cell Lysate Lane 6: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH at 1/10000 dilu
Sample: Lane 1,4,7: Mouse Cerebellum tissue lysates Lane 2,5,8: Rat Cerebellum tissue lysates Lane 3,6,9: Human SH-SY5Y cell lysates Primary: Anti-GAPDH at 1/10000~1/100000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to th
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP K
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation t
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-R
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat Anti
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C. Followed by conjugated Goat Anti
GAPDH was immunoprecipitated from Jurkat cell lysate with abs132004 at 1/200 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti-Rabbit IgG (H&L) was used as secondary antibody. Lane 1: Jurkat
文献相关图
(A) Protein expression levels of p‑AMPK, AMPK, Occludin, Claudin‑3, ZO‑1 and BSEP were determined by western blotting in Sham, Control and FO‑treated mice on days 1, 2, 3 and 5 following partial hepatectomy; β‑actin was used as an internal Control.
来源于《J Cell Physiol》
Western blot检测结果;1:L-MC mock组;2:L-MC pcDNA3.1组;3:L-MC Rpph1(+)组;4:H-MC mock组
来源于《Chinese Journal of Osteoporosis》
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