Rabbit anti-E2F1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (E2F1) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjuga

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (E2F1) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjuga

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: A673 (Human) Cell Lysate at 30 ug Lane 3: Molt-4 (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Primary: Anti-E2F1 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbi