Rabbit anti-CD21 Recombinant Monoclonal Antibody(007-47)

WB result of CD21 Rabbit mAb Primary antibody: CD21 Rabbit mAb at 1/1000 dilution Lane 1: K562 whole cell lysate 20 µg Lane 2: Daudi whole cell lysate 20 µg Lane 3: Raji whole cell lysate 20 µg Negative control: K562 whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 113 kDa Observed MW: 160 kDa Exposure time: 20s

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD21 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD21 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative tissue: IHC shows negative staining in paraffin-embedded human kidney. Anti-CD21 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD21 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD21 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows membrane staining in Ramos cells. Anti-CD21 antibody was used at 1/500 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution (shown in green).The cells were fixed with 100% Methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.

Flow cytometric analysis of Raji cells labelling CD21 antibody at 1/500 dilution (0.1ug)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #abs20025 at 1/1000 dilution was used as the secondary antibody.