Rabbit anti-CD11c Recombinant Monoclonal Antibody(396-48)

WB result of CD11c Rabbit mAb Primary antibody: CD11c Rabbit mAb at 1/1000 dilution Lane 1: THP-1 whole cell lysate 20 ug Lane 2: THP-1 serum starvation 2 hour then treated with Phorbol-12-myristate-13-acetate (100ng/ml, 96 h) whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 128 kDa Observed MW: 145kDa Exposure time: 60 s (This blot was developed with high sensitivity substrate)

CD11c Rabbit mAb at 1/50 dilution (1 ug) immunoprecipitating CD11c in 0.4 mg THP-1 serum starvation 2 hours then treated with Phorbol-12-myristate-13-acetate (100ng/ml, 96 h) whole cell lysate. Western blot was performed on the immunoprecipitate using CD11c Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: THP-1 serum starvation 2 hours then treated with Phorbol-12-myristate-13-acetate (100ng/ml, 96 h) whole cell lysate 20 ug (Input) Lane 2: CD11c Rabbit mAb IP in THP-1 serum starvation 2 hours then treated with Phorbol-12-myristate-13-acetate (100ng/ml, 96 h) whole cell lysate Lane 3: Rabbit monoclonal IgG IP in THP-1 serum starvation 2 hours then treated with Phorbol-12-myristate-13-acetate (100ng/ml, 96 h) whole cell lysate Predicted MW: 128 kDa Observed MW: 145 kDa Exposure time: 60 s

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD11c antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD11c antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-CD11c antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD11c antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CD11c antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.