Rabbit anti-β-Actin Recombinant Monoclonal Antibody
| 货号-规格 | 货期 | 价格 | 数量 |
| abs171817-100uL | 1-2周 | ￥900.00 | - + |
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| WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20ug Lane 2: A549 whole cell lysate 20ug Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 42kDa Observed MW: 42kDa Exposure time: 30s | ||
| WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20ug Lane 2: mouse kidney lysate 20ug Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 42kDa Observed MW: 42kDa Exposure time: 30s | ||
| IHC shows positive staining in paraffin-embedded human colon. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
| IHC shows positive staining in paraffin-embedded human colon cancer. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
| IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
| IHC shows positive staining in paraffin-embedded mouse kidney. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
| IHC shows positive staining in paraffin-embedded rat kidney. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
| ICC shows positive staining in HeLa cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red). | ||
| ICC shows positive staining in NIH/3T3 cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red). | ||
| Flow cytometric analysis of HeLa cells labelling β-actin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #abs20025. | ||
| Flow cytometric analysis of NIH/3T3 cells labelling β-actin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #abs20025. | ||
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Certificate of Analysis(COA)
危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS) 
营业执照(三证合一)