Rabbit Anti-beta Actin Polyclonal Antibody
- β Actin抗体;Beta Actin, beta-Actin, ACTB, Actin cytoplasmic 1, Actin, beta, Beta actin, beta cytoskeletal actin,A X actin like protein, ACTB, Actin cytoplasmic 1, alpha sarcomeric Actin, Actx, Beta cytoskeletal actin, Melanoma X actin, PS1TP5BP1, ACTB_HUMAN. , ,
货号： abs119600
| 货号-规格 | 货期 | 价格 | 数量 |
| abs119600-50ul | 1-2周 | ￥1500.00 | - + |
| abs119600-100ul | 1-2周 | ￥2300.00 | - + |
大包装询价| 概述 | ||
| 别名 | β Actin抗体;Beta Actin, beta-Actin, ACTB, Actin cytoplasmic 1, Actin, beta, Beta actin, beta cytoskeletal actin,A X actin like protein, ACTB, Actin cytoplasmic 1, alpha sarcomeric Actin, Actx, Beta cytoskeletal actin, Melanoma X actin, PS1TP5BP1, ACTB_HUMAN. , , | |
| 宿主 | Rabbit | |
| 特异性 | beta-Actin (Loading Control) | |
| 反应种属 | Human;Mouse;Rat;mt;op | |
| 预测反应种属 | Chicken;Dog;Pig;Rabbit;Sheep;Bee;Fish;GuineaPig;Hamster;Cat; | |
| 应用 | WB=1:5000-50000, Flow-Cyt=1μg/Test, ICC=1:100 | |
| 分子量 | Predicted molecular weight: 42kDa | |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin:1-200/375 | |
| 性能 | ||
| 浓度 | 1mg/ml | |
| 纯化方法 | affinity purified by Protein A | |
| 类型 | Polyclonal Antibody | |
| 同种型 | IgG | |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. | |
| 存储溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. | |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞骨架 | |
| 靶标 | ||
| 背景说明 | Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. | |
| 细胞定位 | 细胞浆
| |
| UniProt | ||
| Sample: A549 Cell (Human) Lysate at 30 ug Primary: Lane1: Anti-beta-actin at 1/500 dilution Lane2: Anti-beta-actin at 1/1000 dilution Lane3: Anti-beta-actin at 1/2000 dilution Lane4: Anti-beta-actin at 1/4000 dilution Lane5: Anti-beta-actin at 1/5000 dilution Lane6: Anti-beta-actin at 1/8000 dilution Lane7: Anti-beta-actin at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 47 kD | ||
| Sample: A549 Cell (Human) Lysate at 30 ug Primary: Lane1: Anti-beta-actin at 1/500 dilution Lane2: Anti-beta-actin at 1/1000 dilution Lane3: Anti-beta-actin at 1/2000 dilution Lane4: Anti-beta-actin at 1/4000 dilution Lane5: Anti-beta-actin at 1/5000 dilution Lane6: Anti-beta-actin at 1/8000 dilution Lane7: Anti-beta-actin at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 47 kD | ||
| Sample: Lane1: 293T Cell Lysate at 25 ug Lane2: A549 Cell Lysate at 25 ug Lane3: A431 Cell Lysate at 25 ug Primary: Anti- beta-Actin at 1/1000 and 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 42 kD | ||
| Sample: Lung lysate at 30ug; Primary: Anti-beta-actin at 1:1000 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution Predicted band size : 42kD Observed band size : 42kD | ||
| Blank control: RSC96(blue). Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed. | ||
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
- 实验方法 实验条件
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