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  • Rabbit Anti-beta Actin Polyclonal Antibody

    • β Actin抗体;Beta Actin, beta-Actin, ACTB, Actin cytoplasmic 1, Actin, beta, Beta actin, beta cytoskeletal actin,A X actin like protein, ACTB, Actin cytoplasmic 1, alpha sarcomeric Actin, Actx, Beta cytoskeletal actin, Melanoma X actin, PS1TP5BP1, ACTB_HUMAN. , ,
    货号: abs119600
    产品说明书
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    货号-规格 货期 价格 数量
    abs119600-50ul 1-2周 ¥1500.00
    - +
    abs119600-100ul 1-2周 ¥2300.00
    - +
    大包装询价
    概述
    别名
    β Actin抗体;Beta Actin, beta-Actin, ACTB, Actin cytoplasmic 1, Actin, beta, Beta actin, beta cytoskeletal actin,A X actin like protein, ACTB, Actin cytoplasmic 1, alpha sarcomeric Actin, Actx, Beta cytoskeletal actin, Melanoma X actin, PS1TP5BP1, ACTB_HUMAN. , ,
    宿主
    Rabbit
    特异性
    beta-Actin (Loading Control)
    反应种属
    Human;Mouse;Rat;mt;op
    预测反应种属
    Chicken;Dog;Pig;Rabbit;Sheep;Bee;Fish;GuineaPig;Hamster;Cat;
    应用
    WB=1:5000-50000, Flow-Cyt=1μg/Test, ICC=1:100
    分子量
    Predicted molecular weight: 42kDa
    免疫原
    Synthetic MAP peptide derived from human beta-Actin:1-200/375 
    性能
    浓度
    1mg/ml
    纯化方法
    affinity purified by Protein A
    类型
    Polyclonal Antibody
    同种型
    IgG
    储存/保存方法
    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    存储溶液
    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    研究领域
    肿瘤  细胞生物  信号转导  细胞骨架  
    靶标
    背景说明
    Loading Control

    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].
    细胞定位
    细胞浆 
    UniProt
    Sample:
    A549 Cell (Human) Lysate at 30 ug
    Primary: Lane1: Anti-beta-actin at 1/500 dilution
    Lane2: Anti-beta-actin at 1/1000 dilution
    Lane3: Anti-beta-actin at 1/2000 dilution
    Lane4: Anti-beta-actin at 1/4000 dilution
    Lane5: Anti-beta-actin at 1/5000 dilution
    Lane6: Anti-beta-actin at 1/8000 dilution
    Lane7: Anti-beta-actin at 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 47 kD
    Sample:
    A549 Cell (Human) Lysate at 30 ug
    Primary: Lane1: Anti-beta-actin at 1/500 dilution
    Lane2: Anti-beta-actin at 1/1000 dilution
    Lane3: Anti-beta-actin at 1/2000 dilution
    Lane4: Anti-beta-actin at 1/4000 dilution
    Lane5: Anti-beta-actin at 1/5000 dilution
    Lane6: Anti-beta-actin at 1/8000 dilution
    Lane7: Anti-beta-actin at 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 47 kD
    Sample:
    Lane1: 293T Cell Lysate at 25 ug
    Lane2: A549 Cell Lysate at 25 ug
    Lane3: A431 Cell Lysate at 25 ug
    Primary: Anti- beta-Actin at 1/1000 and 1/5000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42kD
    Observed band size: 42 kD
    Sample: Lung lysate at 30ug;
    Primary: Anti-beta-actin at 1:1000 dilution
    Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution
    Predicted band size : 42kD
    Observed band size : 42kD
    Blank control: RSC96(blue).
    Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.
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    • 实验方法 实验条件
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