中性粒细胞呼吸爆发检测试剂盒
| 货号-规格 | 货期 | 价格 | 数量 |
| abs50002-25T | 现货 | ￥2000.00 | - + |
| abs50002-50T | 现货 | ￥3200.00 | - + |
| abs50002-100T | 1-2周 | ￥5000.00 | - + |
大包装询价| 产品描述 | ||
| 描述 | 中性粒细胞（PMNs）的吞噬作用在抵御细菌、真菌等病原微生物感染过程中发挥重要作用。吞噬作用过程包括几个主要阶段： 趋化，黏附，内吞，胞内氧依赖性（呼吸爆发）和氧非依赖性的杀伤。 正常条件下细菌感染后，机体PMNs被激活，PMNs吞噬和氧化功能增强是机体重要的天然免疫防御机制。因此通过观察PMNs的活化状态可以帮助了解机体细菌感染的状况。 本试剂盒采用流式细胞仪（FMC）检测PMNs功能，用PMA作为高效刺激剂，以二氢若丹明作为荧光底物。中性粒细胞受刺激后产生反应性氧化物，氧化二氢若丹明为可发出荧光的若丹明123。这个反应可以由溶血素终止。产生的反应性氧化物可以用荧光强度来表示。 PMNs活化和机体的天然免疫能力。可用于研究在各种病理情况下如感染、自身免疫疾病、应急、肿瘤、手术等中中性粒细胞的功能改变及药物的效果评价。 该试剂盒不仅用于人，还可以用于小鼠、大鼠、兔、狗、牛等其他种属。 | |
| 产品组分 | PMA溶液
二氢若丹明 溶血素 | |
| 操作步骤 | 1. 无菌肝素抗凝的全血50ul，加刺激剂（如PMA）50ul, 37°C 孵育15分钟。
2．加二氢若丹明25ul, 37°C避光孵育5分钟 3．溶血素用无菌去离子水1：10稀释，加稀释后的溶血素1ml ，室温避光溶血15分钟 4．PBS洗涤2次,1500rpm离心5分钟，去上清液 5．加0.5mlPBS重悬细胞，上流式细胞仪检测 | |
| 基本信息 | ||
| 储存/保存方法 | 详见试剂盒内说明, 避光 | |
| 参考文献 | ||
| 参考文献 | 1 Sawyer, D.W., Donowitz, G.R. & G.L. Mandell. 1989. Polymorphonuclear neutrophils: An effective antimicrobial force. Rev. Infect. Dis. 11: S1532-S1544.
2．Richardson MP, Ayliffe MJ, Helbert M, et al. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods,1998,219(1-2):187-193. 3．Smith, R.M. & J.T. Curnutte. 1991. Molecular basis of chronic granulomatous disease. Blood 77: 673 -686. 4．Jirapongsananuruk O, Malech HL, Kuhns DB, et al. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 2003,111(2):374-9. 5．Walrand S, Valeix S, Rodriguez C, et al. Flow cytometry study of polymorphonuclear neutrophil oxidative burst: a comparison of three fluorescent probes. Clin Chim Acta, 2003, 331(1-2):103-110. 6．Rothe G, Oser A & G. Valet. 1988. Dihydrorhodamin 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften 75: 354 -355. | |
| 前向角、侧向角点状图谱 | ||
| 静止、活化中性粒细胞FL1荧光柱状图谱 | ||
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
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- 试剂盒对全血样本有什么要求吗？
- 建议采血即用，因为全血室温不能超过6小时，如果是冷藏低温会使血液成分和细胞形态发生变化
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危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS)
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