Mouse anti-MonoMethyl-Histone H3(Lys9) Monoclonal Antibody(3F8)

Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human MOLT4 cell lysates Lane 5: Human 293T cell lysates Lane 6: Human U251 cell lysates Primary: Anti-Histone H3 (mono methyl K9)

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C fo

Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30