Mouse anti-CD31 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goa

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-R

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat

Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-

25 ug total protein per lane of various lysates (see on figure) probed with CD31 monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

The THP-1(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD31 antibody : 0.5 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC : 1 μg/test.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugati

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugat

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugat

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugation to

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugation to

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugation to

Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated at 1:1500 overnight at 4°C, followed by conjugation

4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (CD31) monoclonal Antibody, unconjugated 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucl