人淋巴细胞分离液

Histological evaluation of tibiofemoral joints. (A) Histological features of representative tibiofemoral joints sectioned in the sagittal plane stained with HE and toluidine blue. Mankin and OARSI histological scores are shown for each image. F: femur, T: tibia. (B) Mankin score for tibiofemoral joints. Differences between CG and OAG ( * P < 0.001), OAG vs. OAM, OAH and OAM + BOC-2 groups ( + P <0.001), and between OAM and OAM + BOC-2 ( P <0.001) were significant. (C)OARSI histological scores for cartilage of tibiofemoral joints. Differences between CG and OAG ( * P< 0.001), OAG vs. OAL, OAM, OAH and OAM +BOC-2 groups( + P < 0.001) were significant, and between OAM and OAM + BOC-2 ( P < 0.001) were significant. Kruskal-Wallis test, n= 10 rats in each group, means with 95% confidence interval.

The results of ELISA in formal treadmill exercise. The results of ELISA in formal treadmill exercise included LXA 4 (A,D), TNF-α (B,E) and IL-1β (C,F).Differences between CG and OAG were significant ( * P < 0.001), and differences between the OAG group and treadmill exercise groups of different intensities were significant ( + P < 0.001,+a P = 0.010, +b P= 0.009, +c P = 0.014, +d P = 0.002, +e P = 0.042, +f P =0.025, +g P = 0.020, +h P = 0.022), and differences between OAM and OAM + BOC-2 were significant ( P < 0.001,a P= 0.01, b P = 0.013, c P =0.001). One-way ANOVA, n = 10 rats for each group, means with 95% confidence interval.

Immunohistochemical staining in each group. Effect of treadmill exercise with different intensities on the expression of COL2A1, MMP-13 and MMP-3 in knee joint cartilage. The micrographs showed the relative intensity of immunohistochemical staining of COL2A1, and the percentages of positively stained cells of MMP-13 and MMP-3 in the articular cartilage of each experimental group. Differences between CG and OAG were significant ( * P <0.001), and differences between OAG and the treadmill exercise groups were significant ( + P < 0.001,+a P =0.045, +b P= 0.012), and differences between OAM and OAM + BOC-2 were significant( P < 0.001). One-way ANOVA, n = 5 rats for each group, mean score with 95% confidence interval.

XPD siRNA promotes Cyclin D1, Bcl-2, C-sis and reduces P21, Mdm2, Mdm4, Bax, and P53 expression. (C) Representative blots of the related proteins; (D) Quantification data of protein expression. Compared with NC, * P<0.05; Compared with XPD siRNA, P<0.05.

No changes in weight between groups throughout the experiment, N = 15 per group.

HBOT produced a long antinociceptive effect affecting the mechanical withdrawal threshold (MWT). N = 15 per group. ***P < 0.001 vs. Control group,***P < 0.05 vs. PTX group.

GFAP staining for astrocytes expression in the dorsal horn of the spinal cord among groups, N = 5 per group. **P < 0.01 vs. Control group, P < 0.01 vs. PTX group.

The expression of GFAP(a), IL-1 β (b), TNFα (c) in the dorsal horn of the spinal cord among groups, N = 5 per group. **P < 0.01, ***P < 0.001 vs. Control group, P < 0.05 P < 0.01 vs. PTX group.

The mRNA levels of GFAP(a), IL-1 β (b), TNFα (c) in the dorsal horn of the spinal cord among groups, N = 5 per group. +P = 0.0467 vs. Control group, ***P < 0.001 vs. Control group, P < 0.01 vs. PTX group.