Human IFN-γ ELISA Kit(Faster)

Fig. 3.Anti-tumor activity of HIF-1α-overexpressing CAR-T cells under normoxic and hypoxic conditions. (A). Cytotoxicity of UT, CAR-T, and HIF1AOE-CAR- T cells against SKBR3 cells was assessed by LDH release assay at an E:T ratio of 5:1 for 24h under normoxia (21% O2) and hypoxia (1% O2). (B-F) ELISA quan tification of cytokine release following 24h of co-culture with SKBR3 cells under normoxia and hypoxia. (B) IL-2 (C) IFN-γ (D) TNF-α