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  • GST-Tag Polyclonal Antibody
    货号: abs118940
    产品说明书
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    货号-规格 货期 价格 数量
    abs118940-50ul 1-2周 ¥1500.00
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    大包装询价
    概述
    宿主
    Rabbit
    反应种属
    All
    应用
    WB 1:1000 - 1:2000
    IF 1:20 - 1:50
    IP 1:20 - 1:50
    免疫原
    Recombinant protein of human GST-Tag
    阳性对照
    GST fusion protein
    性能
    纯化方法
    Affinity purification
    同种型
    IgG
    储存/保存方法
    Store at -20℃. Avoid freeze / thaw cycles.
    存储溶液
    Store at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    靶标
    背景说明
    Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.
    UniPort
    Western blot analysis of GST-tagged protein using GST antibody at different dilution. Each lane was loaded with 5 ng protein.
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