Goat anti-Rabbit IgG-FITC Antibody

Effect of low-dose PTX on the expression level of glycolysis-related genes in HCT116 cells. HCT116 cells were exposed to PTX at 0.1 and 0.3 nM for 0, 1, 2, or 3 days, followed by gene expression levels detected by quantitative real-time PCR assay and Western blot. (A,D) The mRNA expression of HK1 and PHGDH genes. (B,E) The protein expression of HK1 and PHGDH in cells treated with 0.1 and 0.3 nM PTX for 3 days. (C,F) Quantification of the protein expression of HK1 and PHGDH. Data represent the means ± SD, n = 3 independent experiments. ∗p < 0.05 and ∗∗p < 0.01 versus control.

Effects of low-dose PTX on mRNA expression of glutaminolysis-related genes in HCT116 cells. HCT116 cells were treated with 0.1 and 0.3 nM PTX for 0, 1, 2, or 3 days, and then mRNA and protein expression of glutaminolysis-related genes was detected by quantitative real-time PCR assay and Western blot. (A,D,G,J) The mRNA expression of GLS, SLC1A5, SLC7A11 and GLUD1 genes. (B,E,H,K) The protein expression of GLS, SLC1A5, SLC7A11 and GLUD1 in cells treated with 0.1 and 0.3 nM PTX for 3 days. (C,F,I,L) Quantification of the protein expression, GLS, SLC1A5, SLC7A11 and GLUD1. Data represent the means ± SD, n = 3 independent experiments. ∗p < 0.05 and ∗∗p < 0.01 versus control.

Berberinepromotesglucoseuptakeanditssub-toxiceffectoncellviability.A.KGNcellswereseededinto96-wellplatesat103cells/wellinthepresenceof berberineatconcentrationsrangingfrom0to200μMfor24h.TheeffectsofberberineoncellgrowthandproliferationweredeterminedbytheCCK8method.B. KGNcellswereseededinto24-wellplatesandincubatedwithberberineatconcentrationsof0–50μMfor24h.Aftertreatment,thecellswereincubatedfor1hwith 100μM 2-NBDG and 100nM insulin. Intracellular green fluorescence intensities at different berberine concentrations were assessed by fluorescence microscopy. Flowcytometryanalysisofintracellularfluorescenceintensityisdepictedashistograms.C.IntracellularmediangreenfluorescenceintensitiesofKGNcellstreated withberberinewereassessedbyflowcytometry.Dataaremean±SEM. P<0.05;*P<0.01.