Fluc mRNA(N1-Methylpseudo-UTP)

Fig. 1. mRNA transfection and biodistribution of peptide modified LNPs. (A) Luciferase expression in GL261 cells treated with Luc mRNA LNPs containing 10% or 30% peptide modified lipid-PEG of total lipid-PEG (n = 3). (B) Quantification of luminescence intensity in mouse brains (n = 3). (C) Brain to liver luminescence ratios after intravenous injections with Luc mRNA LNPs or Luc mRNA RLNPs. Luc mRNA LNPs have 0% peptide modified lipid-PEG substitution, Luc mRNA RLNPs have 10% RGD modified lipid-PEG substitution (n = 3). (D) Representative IVIS bioluminescence images of organs from mice treated with Luc mRNA LNPs or Luc mRNA RLNPs (n = 3). (E) Distribution of RLNPs in brain sections of tumor-bearing and healthy mice. Scale bar is 100 μm. Data are presented as mean ± SD. Statistical significance was analyzed by two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.