Daporinad

NaBu suppresses HG‑induced NRK‑52E cells apoptosis. (A) The influence of different concentrations of NaBu (0.1, 0.5 and 1.0 mmol/l) on cell viability was detected by CCK8 assay. (B) Caspase‑3 activity in different experimental groups. (C) Expression of Bax and Bcl‑2 was analyzed by western blotting. (D) Flow cytometry analysis of apoptosis in NRK‑52E cells. Cells in right upper quadrant were defined as late apoptotic cells, and cells in lower right quadrant were defined as early apoptotic cells. Cells in the above two quadrants were considered apoptotic cells. Data are presented as the mean ± standard deviation (n=3). **P<0.01 vs. NG group; P<0.05, P<0.01 vs. HG group. NaBu, sodium butyrate; HG, high glucose; NG, normal glucose.

NaBu suppresses HG‑induced NRK‑52E cells apoptosis. (A) The influence of different concentrations of NaBu (0.1, 0.5 and 1.0 mmol/l) on cell viability was detected by CCK8 assay. (B) Caspase‑3 activity in different experimental groups. (C) Expression of Bax and Bcl‑2 was analyzed by western blotting. (D) Flow cytometry analysis of apoptosis in NRK‑52E cells. Cells in right upper quadrant were defined as late apoptotic cells, and cells in lower right quadrant were defined as early apoptotic cells. Cells in the above two quadrants were considered apoptotic cells. Data are presented as the mean ± standard deviation (n=3). **P<0.01 vs. NG group; P<0.05, P<0.01 vs. HG group. NaBu, sodium butyrate; HG, high glucose; NG, normal glucose.