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  • Blebbistatin

    • (-)-Blebbistatin;(S)-(-)-Blebbistatin
    货号: abs813522
    CAS号: 856925-71-8
    分子式: C18H16N2O2
    分子量: 292.33
    产品说明书
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    货号-规格 货期 价格 数量
    abs813522-5mg 1-2周 ¥717.00
    - +
    abs813522-10mg 1-2周 ¥1171.00
    - +
    abs813522-20mg 1-2周 ¥2205.00
    - +
    abs813522-50mg 1-2周 ¥4631.00
    - +
    大包装询价
    产品描述
    描述

    (-)-Blebbistatin是一种可渗透细胞的抑制剂,作用于非肌球蛋白IIATPase,IC50为2 μM,不抑制肌球蛋白轻链激酶,抑制卵裂沟的缢缩,不干扰有丝分裂或收缩环的组装。Blebbistatin抑制细胞分裂,改变鱼角膜细胞的平滑运动,且抑制缺乏细丝蛋白的细胞系进行自发起泡。Blebbistatin抑制非肌肉肌球蛋白IIA,非肌肉肌球蛋白IIB,和兔骨骼肌肌球蛋白S1的HMM片段的酶活性,而不抑制平滑肌球蛋白。Blebbistatin快速且可逆抑制Mg-ATPase活性,也抑制非肌肉肌球蛋白IIA和IIB的体外活性,而极其微弱抑制平滑肌肌球蛋白(IC50=80 μM)。

    纯度
    >98%
    储存/保存方法
    Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
    基本信息
    别名
    (-)-Blebbistatin;(S)-(-)-Blebbistatin
    可溶性/溶解性
    DMSO :29.2 mg/mL (100 mM)
    生物活性
    靶点
    Myosin
    In vitro(体外研究)
    Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC50=80 μM). Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca2+ release. It promotes wound-induced cell migration.
    In vivo(体内研究)
    Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz.
    温馨提示:本产品仅作科研实验使用,不支持临床等研究
    • 实验方法 实验条件
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