BCA 蛋白定量试剂盒
| 货号-规格 | 货期 | 价格 | 数量 |
| abs9232-500次 | 现货 | ￥437.00 | - + |
| abs9232-2500次 | 1-2周 | ￥1142.00 | - + |
大包装询价| 产品描述 | |||||||||||||
| 描述 | BCA法是理想的蛋白质定量方法，在碱性环境下蛋白质分子中肽键结构与Cu2+络合并将Cu2+还原成Cu1+。BCA特异地与Cu1+结合形成稳定的紫蓝色复合物，在562nm处有最大的光吸收值并与蛋白质浓度成正比，颜色的深浅与蛋白质的含量成正比，可以根据吸收值测定蛋白质浓度。该测定方法灵敏度高，操作简单，试剂及其形成的颜色复合物稳定性俱佳，并且受干扰物质去垢剂等影响小。 产品特点： 1、准确灵敏，线性范围广，BCA试剂的蛋白质测定范围是20~2000ug/mL； 2、快速：45min内完成测定，比经典的Lowry法快4倍而且更加方便； 3、不受样品中离子型和非离子型去污剂影响； 4、检测不同蛋白质分子的变异系数远小于考马氏亮蓝,可以酶标板法或分光光度计法测定蛋白质浓度。 组分：
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| 注意事项 | 1、将A、B液恢复到室温再使用，有利于提高检测的灵敏度。若A液出现物质析出，请室温平衡后，轻轻晃动，使其溶解后再使用。 2、蛋白标准请混匀后，再稀释成一系列不同浓度的蛋白标准。 3、需酶标仪一台，最佳检测波长为562nm。并需96孔板。 | ||||||||||||
| 基本信息 | |||||||||||||
| 储存/保存方法 | BCA蛋白定量试剂盒A、B应保存于2~8℃；5mg/mL BSA应保存于-20℃。有效期12个月。 | ||||||||||||
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
- 实验方法 实验条件
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