文献标题：Mutant p53 epigenetically rewires CXCL10 to promote CD8⁺ T-cell infiltration and enhance the anti-PD-1 response in advanced prostate cancer

发表期刊：J Exp Clin Cancer Res. (IF=12.8)

DOI：https://doi.org/10.1186/s13046-026-03672-z

使用 Absin 产品：DNase I（货号：abs60539）、胶原酶 Ⅳ型（货号：abs47048003）

Fig. 2. Mutant p53 reprograms epithelial subpopulations and metabolism to promote tumor progression. a UMAP plots showing reclustering of epithelial cells from WTp53 and Mutp53 tumors into seven distinct transcriptional states (C0-C6). b Box plots showing the relative abundance of each epithelial cluster by genotype; C0, C2, and C6 are enriched in Mutp53, whereas C3-C5 are reduced (p values as indicated, two-sided Wilcoxon rank-sum test). c Ro/e enrichment scores indicating relative expansion (≥ 1.0) or depletion (< 1.0) of each cluster in WTp53 versus Mutp53 tumors. dHeatmap of cluster-dominant genes with associated Gene Ontology (GO, left) and KEGG pathway (right) enrichment. e Monocle 3 trajectory analysis showing pseudotime inference, with C0, C2, and C6 positioned at terminal states, which is consistent with malignant progression. f Violin plots displaying significantly elevated arachidonic acid and fructose/mannose metabolism scores in Mutp53 epithelial cells (both p ≤ 2 × 10−¹⁶, Wilcoxon rank-sum test). g CCK-8 proliferation assays demonstrating that pharmacological inhibition of fructose metabolism (LY3522348) or arachidonic acid metabolism (glafenine) selectively suppressed Mutp53 cell growth but minimally affected WTp53 cells (two-way ANOVA followed by Tukey’s post hoc pairwise comparisons at 72 h). h Representative images of EdU-stained WTp53 and Mutp53 cells after metabolic inhibitor treatment at 48 h. Scale bars, 50 μm. i Quantification of the significant reduction in the proliferation of the Mutp53 cells upon inhibitor treatment compared with that of the control cells (one-way ANOVA followed by Tukey’s post hoc pairwise comparisons). Note: Data are presented as the mean ± SEM. Significance is indicated as *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Abbreviations: UMAP, uniform manifold approximation and projection; Ro/e, observed-to-expected ratio; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; scMetabolism, single-cell metabolism analysis; SEM, standard error of the mean