Mouse anti-Myc Tag Monoclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs149605-50uL | 1-2周 | ¥800.00 | - + |
abs149605-100uL | 现货 | ¥1200.00 | - + |
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Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate. | ||
Immunoprecipitation of Myc-tagged protein from HEK293T cells transfected with vector overexpressing Myc tag, using Anti-Myc-tag Antibody. | ||
Immunofluorescent analysis of Myc-tag staining in 293T cells transfected with a Myc-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). | ||
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