Rabbit anti-SOX2 Recombinant Monoclonal Antibody(066-58)

WB result of sox2 Rabbit mAb Primary antibody: sox2 Rabbit mAb at 1/1000 dilution Lane 1: NCCIT lysate 20 µg Lane 2: MCF7 bladder lysate 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 35 kDa Exposure time: Lane 1: 2s Lane 2: 40s

IHC shows positive staining in paraffin-embedded human stomach. Anti-SOX2 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung. Anti-SOX2 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer.Anti-SOX2 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human seminoma. Anti-SOX2 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

SOX2 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating SOX2 in 0.4mg NCCIT whole cell lysate. Western blot was performed on the immunoprecipitate using SOX2 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: NCCIT whole cell lysate 10µg (input) Lane 2: SOX2 Rabbit mAb IP in NCCIT whole cell lysate Lane 3: Rabbit monoclonal IgG IP in NCCIT whole cell lysate Predicted MW: 35 kDa Observed MW: 35 kDa Exposure time: 1.64s