Rabbit anti-Myosin-11 Recombinant Monoclonal Antibody(188-112)

WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: mouse skeletal muscle lysate 5ug Lane 2: mouse bladder lysate 5ug Negative control: mouse skeletal muscle lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s

WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/1000 dilution Lane 1: mouse skeletal muscle lysate 20ug Lane 2: mouse prostate lysate 20ug Negative control: mouse skeletal muscle lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 60s

WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: rat skeletal muscle lysate 5ug Lane 2: rat bladder lysate 5ug Negative control: rat skeletal muscle lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s

IHC shows positive staining in paraffin-embedded human breast. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IF shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Secondary antibody: #abs20025 at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded mouse colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Secondary antibody: #abs20025 at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.