Rabbit anti-CytokeRatin 7 Recombiant Monoclonal Antibody(121-17)

WB result of Cytokeratin 7 Rabbit mAb Primary antibody: Cytokeratin 7 Rabbit mAb at 1/1000 dilution Lane 1: MCF7 whole cell lysate 20 µg Lane 2: A549 whole cell lysate 20 µg Lane 3: Hela whole cell lysate 20 µg Lane 4: SK-BR-3 whole cell lysate 20 µg Negative control: MCF7 whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 40~55 kDa Observed MW: 40~55 kDa Exposure time: 40s

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast. Anti-Cytokeratin 7 antibody was used at 1/2000 dilution,Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma cancer. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, fSecondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Cytokeratin 7 antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows cytoplasm staining in HeLa cells. Anti-Cytokeratin 7 antibody was used at 1/1000 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% Methonal and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.

Negative control: ICC shows negative staining in MCF7 cells. Anti-Cytokeratin 7 antibody was used at 1/1000 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% Methonal and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.

Flow cytometric analysis of MCF7 (Human monocytic leukemia monocyte, left) / HeLa (Human T cell leukemia T lymphocyte, right) cells labelling Cytokeratin 7 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #abs20025 at 1/1000 dilution . Negative control: MCF7

Cytokeratin 7 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Cytokeratin 7 in 0.4mg SK-BR-3 whole cell lysate. Western blot was performed on the immunoprecipitate using Cytokeratin 7 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: SK-BR-3 whole cell lysate 10µg (input) Lane 2: Cytokeratin 7 Rabbit mAb IP in SK-BR-3 whole cell lysate Lane 3: Rabbit monoclonal IgG IP in SK-BR-3 whole cell lysate Predicted MW: 40~55 kDa Observed MW: 40~55 kDa Exposure time: 0.5s