Rabbit anti-CD3 epsilon Recombinant Monoclonal Antibody(241-49)

WB result of CD3 epsilon Rabbit mAb Primary antibody: CD3 epsilon Rabbit mAb at 1/500 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 20 kDa Observed MW: 20 kDa Exposure time: 13s

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-CD3 epsilon antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD3 epsilon antibody was used at 1/4000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows positive staining in Jurkat cells. Anti-CD3 epsilon antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

Negative control: ICC shows negative staining in Raji cells. Anti-CD3 epsilon antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

CD3 epsilon Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating CD3 epsilon in 0.4mg Jurkat whole cell lysate. Western blot was performed on the immunoprecipitate using CD3 epsilon Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1 : Jurkat whole cell lysate 10µg(input) Lane 2 : CD3 epsilon Rabbit mAb IP in Jurkat whole cell lysate Lane 3 : Rabbit monoclonal IgG IP in Jurkat whole cell lysate Predicted MW: 20 kDa Observed MW: 20 kDa Exposure time: 10s