Rabbit anti-CD14 Recombinant Monoclonal Antibody(060-50)

WB result of CD14 Rabbit mAb Primary antibody: CD14 Rabbit mAb at 1/1000 dilution Lane 1: Neuro-2a whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Lane 3: A549 whole cell lysate 20 µg Lane 4: HL-60 whole cell lysate 20 µg Negative control: Neuro-2a whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 40 kDa Observed MW: 47~57 kDa Exposure time: Lane 1、lane 2 and lane 3: 120s Lane 4: 30s

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD14 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD14 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-CD14 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CD14 antibody was used at 1/2000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Flow cytometric analysis of THP-1 cells labelled with CD14 antibody at 1/50 dilution (1 μg)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody

CD14 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD14 in 0.4mg A549 whole cell lysate. Western blot was performed on the immunoprecipitate using CD14 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1 : A549 whole cell lysate 10µg (input) Lane 2 : CD14 Rabbit mAb IP in A549 whole cell lysate Lane 3 : Rabbit monoclonal IgG IP in A549 whole cell lysate Predicted MW: 40 kDa Observed MW: 47~57 kDa Exposure time: 180s