Rabbit anti-Arginase-1 Recombinant Monoclonal Antibody(369-33)

IHC shows positive staining in paraffin-embedded human liver. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining on immune cells in paraffin-embedded human gastric carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining on immune cells in paraffin-embedded human cervical squamous cell carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Arginase-1 Rabbit mAb at 1/25 dilution (1 ug) immunoprecipitating Arginase-1 in 0.4 mg HepG2 whole cell lysate. Western blot was performed on the immunoprecipitate using Arginase-1 Rabbit mAb at 1/500 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: HepG2 whole cell lysate 20 ug (Input) Lane 2: Arginase-1 Rabbit mAb IP in HepG2 whole cell lysate Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate Predicted MW: 35 kDa Observed MW: 37 kDa Exposure time: 180 s

WB result of Arginase-1 Rabbit mAb Primary antibody: Arginase-1 Rabbit mAb at 1/500 dilution Lane 1: A549 whole cell lysate 40 ug Lane 2: HepG2 whole cell lysate 40 ug Negative control: A549 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 37 kDa Exposure time: 180 s