7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) is a thiol-reactive fluorescent probe. CPM selectively forms Michael adducts in the presence of hyperreactive sulfhydryls. CPM has been used to monitor the release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

A thiol-reactive fluorescent probe

Desiccate at -20° C

Solid

CPM

~1.3 g/cm³ (Predicted)

282.76° C (Predicted)

~618.7° C at 760 mmHg (Predicted)

λex 391 nm, λem 472 nm in methanol

n²⁰_D 1.65 (Predicted)

Soluble in water (partly), methanol, DMSO, DMF, and chloroform.

MFCD00077334

122042