Rabbit anti-TRAIL Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs121750-50uL | 1-2周 | ¥1500.00 | - + |
abs121750-100uL | 1-2周 | ¥2300.00 | - + |
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Sample: Lung (Mouse) Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: rabbit Anti- TRAIL (abs121750) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1:5000 dilution; Predicted band size: 31 kD Observed band size: 26 kD | ||
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
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