Rabbit anti-Synaptopodin Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs124693-50uL | 1-2周 | ¥1500.00 | - + |
abs124693-100uL | 1-2周 | ¥2300.00 | - + |
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Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Synaptopodin (abs124693) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 76 kD | ||
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-Synaptopodin (abs124693) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 102 kD | ||
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at RT for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining. | ||
Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: (abs124693); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA | ||
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