Rabbit anti-Nephrin Polyclonal Antibody
| 货号-规格 | 货期 | 价格 | 数量 |
| abs120474-50uL | 1-2周 | ￥1500.00 | - + |
| abs120474-100uL | 1-2周 | ￥2300.00 | - + |
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| Sample: MDCK(Dog) Cell Lysate at 40 ug Primary: Anti-Nephrin (abs120474) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 138 kD Observed band size: 138 kD | ||
| Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
| Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
| Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). | ||
| the cells(293T) were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). | ||
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
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Certificate of Analysis(COA)
危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS) 
营业执照(三证合一)