Rabbit anti-LOX 1 Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs123947-50uL | 1-2周 | ¥1500.00 | - + |
abs123947-100uL | 1-2周 | ¥2300.00 | - + |
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Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti- LOX 1 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. | ||
Positive control: A549 cells Concebtration: 1μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice. | ||
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