Rabbit anti-Integrin β3 Polyclonal Antibody
| 货号-规格 | 货期 | 价格 | 数量 |
| abs119870-50uL | 1-2周 | ￥1500.00 | - + |
| abs119870-100uL | 1-2周 | ￥2300.00 | - + |
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| Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4癈, followed by conjugation to the secondary antibody and DAB staining | ||
| Overlay histogram showing HL 60 cells stained with (abs119870) (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (abs119870) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. | ||
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Certificate of Analysis(COA)
危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS) 
营业执照(三证合一)