Rabbit anti-GAPDH Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs124037-50uL | 1-2周 | ¥800.00 | - + |
abs124037-100uL | 1-2周 | ¥1200.00 | - + |
Sample: Hela Cell (Human) Lysate at 40 ug MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti- GAPDH (abs124037) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD | ||
Sample: Muscle (Rat) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti- GAPDH (abs124037) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD | ||
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- GAPDH (abs124037) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD | ||
Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- GAPDH(abs124037)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD | ||
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining. | ||
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining. | ||
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
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