Rabbit anti-Fibronectin Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs120115-50uL | 现货 | ¥1500.00 | - + |
abs120115-100uL | 1-2周 | ¥2300.00 | - + |
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Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated secondary primary antibody 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated secondary primary antibody 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (Fibronectin/FN1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. | ||
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Incubation: Anti-Fibronectin Antibody, conjugated 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. | ||
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Incubation: Anti-Fibronectin Polyclonal Antibody, conjugated 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. | ||
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