Rabbit anti-Desmin Recombinant Monoclonal Antibody(R022)
货号-规格 | 货期 | 价格 | 数量 |
abs130084-100uL | 1-2周 | ¥2100.00 | - + |
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WB result of Desmin Rabbit mAb Primary antibody: Desmin Rabbit mAb at 1/10000 dilution Lane 1: C2C12 whole cell lysate 20 µg Lane 2: mouse skeletal muscle lysate 20 µg Lane 3: mouse heart lysate 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 53 kDa Observed MW: 53 kDa Exposure time: 0.5s | ||
WB result of Desmin Rabbit mAb Primary antibody: Desmin Rabbit mAb at 1/10000 dilution Lane 1: rat skeletal muscle lysate 20 µg Lane 2: rat heart lysate 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 53 kDa Observed MW: 53 kDa Exposure time: 0.5s | ||
IHC shows positive staining in paraffin-embedded human colon. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human prostate. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
ICC shows cytoplasm staining in C2C12 cells. Anti-Desmin antibody was used at 1/1000 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% Methonal and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI. | ||
Flow cytometric analysis of C2C12 cells labelling Desmin antibody at 1/250 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #abs20025 at 1/1000 dilution was used as the secondary antibody. | ||
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