Rabbit anti-β Amyloid 1-42 Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs119700-50uL | 1-2周 | ¥1500.00 | - + |
abs119700-100uL | 1-2周 | ¥2300.00 | - + |
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-beta-Amyloid(1-42) Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | ||
Positive control: RSC 96 cells Concentration: 1μg/10^6 cells. Isotype control: Cell lines treated with rabbit IgGinstead of the primary antibody to confirm that primary antibody binding is specific. Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody to confirm no background signal produced from secondary antibody alone. | ||
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