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Rabbit anti-β-Actin Polyclonal AntibodyRabbit anti-β-Actin Polyclonal AntibodyRabbit anti-β-Actin Polyclonal AntibodyRabbit anti-β-Actin Polyclonal AntibodyRabbit anti-β-Actin Polyclonal Antibody
  • Rabbit anti-β-Actin Polyclonal Antibody
  • Rabbit anti-β-Actin Polyclonal Antibody
  • Rabbit anti-β-Actin Polyclonal Antibody
  • Rabbit anti-β-Actin Polyclonal Antibody
  • Rabbit anti-β-Actin Polyclonal Antibody
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  • Rabbit anti-β-Actin Polyclonal Antibody

    折上赠
    货号-规格 货期 价格 数量
    abs119600-50uL 1-2周 ￥1500.00
    - +
    abs119600-100uL 1-2周 ￥2300.00
    - +
    大包装询价
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    确定
    产品介绍 商品评论 FAQ 引用文献(13) 相关视频 相关文档 相关产品
    Sample:
    A549 Cell (Human) Lysate at 30 ug
    Primary: Lane1: Anti-beta-actin at 1/500 dilution
    Lane2: Anti-beta-actin at 1/1000 dilution
    Lane3: Anti-beta-actin at 1/2000 dilution
    Lane4: Anti-beta-actin at 1/4000 dilution
    Lane5: Anti-beta-actin at 1/5000 dilution
    Lane6: Anti-beta-actin at 1/8000 dilution
    Lane7: Anti-beta-actin at 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 47 kD
    Sample:
    A549 Cell (Human) Lysate at 30 ug
    Primary: Lane1: Anti-beta-actin at 1/500 dilution
    Lane2: Anti-beta-actin at 1/1000 dilution
    Lane3: Anti-beta-actin at 1/2000 dilution
    Lane4: Anti-beta-actin at 1/4000 dilution
    Lane5: Anti-beta-actin at 1/5000 dilution
    Lane6: Anti-beta-actin at 1/8000 dilution
    Lane7: Anti-beta-actin at 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 47 kD
    Sample:
    Lane1: 293T Cell Lysate at 25 ug
    Lane2: A549 Cell Lysate at 25 ug
    Lane3: A431 Cell Lysate at 25 ug
    Primary: Anti- beta-Actin at 1/1000 and 1/5000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42kD
    Observed band size: 42 kD
    Sample: Lung lysate at 30ug;
    Primary: Anti-beta-actin at 1:1000 dilution
    Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution
    Predicted band size : 42kD
    Observed band size : 42kD
    Blank control: RSC96(blue).
    Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.
    温馨提示：本产品仅作科研实验使用，不支持临床等研究
    • 实验方法 实验条件
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      Certificate of Analysis(COA)

      • Rabbit anti-β-Actin Polyclonal Antibody产品说明书
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