Rabbit anti-LGR5 Polyclonal Antibody(loop2)
| 货号-规格 | 货期 | 价格 | 数量 |
| abs106134-50uL | 1-2周 | ￥1500.00 | - + |
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| Overlay histogram showing SH-SY5Y cells stained with abs106134 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (abs106134, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. | ||
| Overlay histogram showing SH-SY5Y cells stained with abs106134 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (abs106134, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. | ||
| All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilution Lane 1: mouse skeletal muscle lysates Lane 2: rat skeletal muscle lysates Lane 3: Hela whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST. | ||
| All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2whole cell lysates Lane 3: mouse cerebellum lysates Lane 4: mouse skeletal muscle lysates Lane 5: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST. | ||
| Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with abs106134 LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292). | ||
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Certificate of Analysis(COA)
危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS) 
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