即用型高效免疫组化二抗试剂盒
| 货号-规格 | 货期 | 价格 | 数量 |
| abs957-5mL | 现货 | ￥2000.00 | - + |
大包装询价| 组分编号 | 组分名称 | 规格 | 数量 |
| A | 过氧化氢 | 5ml | 1 |
| B | 超级封闭液 | 5ml | 1 |
| C | 一抗放大剂 | 5ml | 1 |
| D | 酶标二抗聚合物 | 5ml | 1 |
| E | DAB A液 | 5ml | 1 |
| F | DAB B液 | 160ul | 1 |
未提供但必需试剂：一抗（来源于小鼠或兔子）、PBS （磷酸盐缓冲液）
本试剂盒是基于聚合物技术的最新一代免疫组化二抗非生物素检测系统，信号更强、操作更加简单。 与传统的免疫组化二抗试剂盒相比，具有3个特点：一是由于聚合物的独到之处，使得酶分子与二抗IgG分子直接结合形成的多聚物分子高度敏感性，有效减少了一抗使用量，一抗试剂可再稀释2-4倍；二是由于避免了生物素的使用，从而可以避免由于生物素引起的非特异性背景显色，得到更清晰的显色和更干净的背景；三是快速，更短的试剂孵育时间。
储存及有效期
2-8°C保存。每个组分至少可以保存18个月。
操作步骤：
说明：所有的步骤在室温下操作，加试剂可以直接滴瓶滴定，也可以用移液器，一滴=30-40ul
1. 脱蜡、水化组织切片
2. PBS洗2-3次各5分钟
3. 根据所应用的一抗的特殊要求，对组织切片进行预处理
4. PBS洗2-3次各5分钟
5. 加入100ul A 液孵育十分钟，阻断内源性过氧化物酶，以减少非特异性背景染色
6. PBS洗2-3次各5分钟
7. 加入100ul B 液孵育五分钟来减少非特异性染色。注意：此步骤不要超过十分钟；如果一抗在含有5％至10％正常山羊血清的缓冲液中稀释，则此步骤可以省略
8. PBS洗2-3次各5分钟
9. 加一抗，室温或37℃孵育20分钟
10. PBS洗2-3次各5分钟
11. 加入100ul C 液孵育十分钟
12. PBS洗2-3次各5分钟
13. 加入100ul D 液孵育十分钟.注意：此液对光敏感，注意避光
14. PBS洗2-3次各5分钟
15. 配制新鲜底物液：将30ul的 F 液和1ml的 E 液充分混合，即配制成新鲜底物液（该底物液可存放2周）
16. 加入100ul新鲜底物液, 孵育五分钟
17. 去离子水冲洗
18. 复染，封片
- 实验方法 实验条件
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- 超级封闭液里面含有triton X-100吗？
- 大概含0.4-0.5%的triton X-100
- 此试剂盒适用于什么种属？
- 一抗为兔和小鼠的均可使用
- 此试剂盒适用于细胞样本吗？
- 不推荐，此试剂盒推荐组织样本。
- 此试剂盒适用于植物样本吗？
- 不推荐，我们都是用动物样本测试的，植物样本没有相关数据，需要客户自行尝试。
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- IL-31在RA患者外周血及关节滑液中的水平及其与关节炎症的关系
钱媛
遵义医科大学. 2020 Jun 30 .
- Abscisic Acid-Induced Autophagy Selectively via MAPK/JNK Signalling Pathway in Glioblastoma
Nan Zhou, Zixuan Wei, Zengxin Qi, Liang Chen
Cellular and Molecular Neurobiology. 2020 Jun 23 .
影响因子： 3.606
- 4EBP1及p4EBP1在前列腺癌临床标本中的表达及其在前列腺癌细胞株中的生物学功能研究
王恺赟
宁波大学. 2020 Jun 08 .
- 薄荷醇缓解胃食管反流的机制研究及技术研发
张志明
江南大学. 2020 Jun 01 .
- SLC26A3转运体（DRA）在溃疡性结肠炎中增强肠上皮细胞屏障的作用研究
丁向明
华中科技大学. 2020 Jun 01 .
- 靶向肿瘤血管的溶瘤腺病毒构建及感染性质分析
李哲
吉林大学. 2020 Jun 01 ; 8
- 双氢睾酮通过TLR4/NF-κB和MAPK信号通路抑制炎性反应对神经元和认知功能的保护作用及机制研究
周任远
天津医科大学. 2020 May 01 .
- 不同配方明胶海绵/纤维蛋白胶支架对关节软骨碎块化后软骨细胞生物活性的影响
雷运亮
南华大学. 2020 May 01 .
- MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivo
Bo Cheng, Qiaofang Wang, Yaodong Song, Yanna Liu, Yanyan Liu, Shujun Yang, Dejian Li, Yan Zhang，Changju Zhu
Scientific Reports. 2020 Apr 21 .
影响因子： 4.379
- VX-765 attenuates atherosclerosis in ApoE deficient mice by modulating VSMCs pyroptosis
Yiyi Li, Xuan Niu, Haitao Xu, Qingwen Li, Lanxia Meng, Mingyang He, Jie Zhang, Zhentao Zhang, Zhaohui Zhang
Experimental Cell Research. 2020 Apr 01 ; 1
影响因子： 3.905
- Screening and Identification of Potential Biomarkers for Hepatocellular Carcinoma: An Analysis of TCGA Database and Clinical Validation
Xianli Wei, Junzi Ke, Haonan Huang, Shikun Zhou, Ao Guo, Kun Wang, Yujuan Zhan, Cong Mai, Weizhen Ao, Fuda Xie, Rongping Luo, Jianyong Xiao, Hang Wei, Bonan Chen
Cancer Manag Res. 2020 Mar 17 .
影响因子： 2.243
- Neonatal exposure to bisphenol A advances pubertal development in female rats
Jing Qiu,Yanyan Sun,Wen Sun,Yonghong Wang,Teng Fan,Jian Yu
Molecular reproduction Development. 2020 Feb 28 ; 4
影响因子： 2.124
- Menthol relieves acid reflux inflammation by regulating TRPV1 in esophageal epithelial cells
Zhiming Zhang, Xiaoli Wu, Liwen Zhang, Aiqin Mao, Xin Ma, Dongxu He
BIOCHEM BIOPH RES CO. 2020 Feb 17 .
影响因子： 2.705
- Laminin α5 modulates fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathway
Pengran Liu, Hui Chen, Lianqi Yan, Yu Sun
Molecular Medicine Reports. 2020 Jan 28 .
- p38α Mitogen-Activated Protein Kinase Is a Druggable Target in Pancreatic Adenocarcinoma
Ling Yang, Xiaoting Sun, Ying Ye, Yongtian Lu, Ji Zuo, Wen Liu, Adrian Elcock, Shun Zhu
Frontiers in Oncology. 2019 Nov 26 .
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- Expression of GLS1 in intrahepatic cholangiocarcinoma and its clinical significance
Jun Cao,Chi Zhang,Guo‑Qing Jiang,Sheng‑Jie Jin,Zhi‑Hui Gao,Qian Wang,De‑Cai Yu,Ai‑Wu Ke,Yi‑Qun Fan,Da‑Wei Li,Ao‑Qing Wang,Dou‑Sheng Bai
Mol Med Rep. 2019 Jun 19 .
影响因子： 1.922
- 乳腺癌患者的D人格与分子分型及预后的相关性分析
张宁
华北理工大学. 2019 May 24 .
- 复方清舒油膏防治急性放射性皮肤损伤的临床疗效观察及作用机制研究
吴华
南京中医药大学. 2019 Mar 18 .
- A7细胞群去甲肾上腺素能神经元对慢性间歇低氧大鼠颏舌肌中枢调控的影响
聂昕时
中国医科大学. 2019 Mar 01 .
- 唾液酸转移酶ST3Gal3在子宫内膜接受态建立中的作用研究
王浩
大连医科大学. 2019 Mar 01 ; 4
- 乳腺癌分子分型与人格特征的关系
张宁;赵月;苏楠;杨乐;李英杰
癌症进展. 2019 Feb 25 .
- lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/β‑catenin pathway in oral squamous cell carcinoma
Fubo Chen, Shengcai Qi, Xu Zhang, Jinjin Wu, Xi Yang, Raorao Wang
International Journal of Oncology. 2019 Feb 01 ; 4
影响因子： 5.65
- Chrysin protects against renal ischemia reperfusion induced tubular cell apoptosis and inflammation in mice
Mingwei Xu,Hongbo Shi,Dongcao Liu
EXP THER MED. 2019 Jan 21 .
影响因子： 1.41
- TMEM206通过与AKT和细胞外信号调节激酶信号通路相互作用促进结直肠癌细胞的恶性表型
赵劲博
中国医科大学. 2018 Aug 01 ; 2
Certificate of Analysis(COA)
危险品化学品经营许可证(不带存储) 许可证编号：沪(浦)应急管危经许[2022]202585(YS) 
营业执照(三证合一)