Rabbit anti-p38 MAPK Recombinant Monoclonal Antibody(S-508-19)
折上赠货号-规格 | 货期 | 价格 | 数量 |
abs131944-100uL | 1-2周 | ¥2100.00 | - + |
WB result of p38 MAPK Rabbit mAb Primary antibody: p38 MAPK Rabbit mAb at 1/5000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: Jurkat whole cell lysate 20 ug Lane 3: MCF7 whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 38 kDa Exposure time: 90 s | ||
WB result of p38 MAPK Rabbit mAb Primary antibody: p38 MAPK Rabbit mAb at 1/5000 dilution Lane 1: NIH/3T3 whole cell lysate 20 ug Lane 2: mouse spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 38 kDa Exposure time: 180 s | ||
WB result of p38 MAPK Rabbit mAb Primary antibody: p38 MAPK Rabbit mAb at 1/5000 dilution Lane 1: C6 whole cell lysate 20 ug Lane 2: rat spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 38 kDa Exposure time: 180 s | ||
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human kidney. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-p38 MAPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol. | ||
ICC shows positive staining in HeLa cells. Anti-p38 MAPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI. | ||
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling p38 MAPK antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor 488 was used as the secondary antibody. | ||
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