Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
Intermembrane side,Mitochondrion inner membrane,Peripheral membrane protein
Western blot analysis of extracts of various cell lines, using CKMT2 antibody (abs118309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat heart tissue using CKMT2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus tissue using CKMT2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart tissue using CKMT2 antibody at dilution of 1:100 (40x lens).