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Rabbit anti-CKII alpha (CSNK2A1) (Center) Polyclonal Antibody

Casein kinase II subunit alpha, CK II alpha, CSNK2A1, CK2A1;CSNK2A1;CK2A1

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货号 abs111567
规格 价格
¥1100
库存状态:4周

数量:

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概述
描述 Purified Rabbit Polyclonal Antibody (Pab)
别名 Casein kinase II subunit alpha, CK II alpha, CSNK2A1, CK2A1;CSNK2A1;CK2A1
来源宿主 Rabbit
特异性 This CKII alpha (CSNK2A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human CKII alpha (CSNK2A1).
应用 WB~~1:2000
IHC-P~~1:50~100
反应种属
Human, Mouse, Rat
性能
保存方法 Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
存储溶液 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
类型 Polyclonal antibody
UniProt P68400
同种型 Rabbit Ig
分子量 45.1 kDa
参考文献
Miyata, Y., et al., Mol. Cell. Biol. 24(9):4065-4074 (2004).
Loizou, J.I., et al., Cell 117(1):17-28 (2004).
Filhol, O., et al., EMBO Rep. 5(4):351-355 (2004).
Kulartz, M., et al., Biochem. Biophys. Res. Commun. 315(4):1011-1017 (2004).
Sachs, N.A., et al., J. Neurochem. 88(1):51-62 (2004).
实验结果图
All lanes : Anti-CSNK2A1 Antibody (Y255) at 1:2000 dilution
Lane 1: C6 whole cell lysates
Lane 2: MOLT-4 whole cell lysates

Lysates/proteins at 20 µg per lane. 

Secondary
Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 45 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-CSNK2A1 Antibody (Y255) at 1:2000 dilution
Lane 1: C6 whole cell lysates
Lane 2: MOLT-4 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 45 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.