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Recombinant Mouse LIF

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货号 abs00998
规格 价格
¥ 720
库存状态:1-2周
¥ 1800
库存状态:1-2周
¥ 19968
库存状态:1-2周

数量:

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概述
种属 Murine
形态 Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.
来源 Escherichia coli.
氨基酸序列 SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
内毒素水平 Less than 1 EU/μg of rMuLIF as determined by LAL method.
活性 Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
Accession # P09056
预期分子量 Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
性能
保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度 > 98 % by SDS-PAGE and HPLC analyses.
Gene IDs 16878
参考文献
1. Eswari S, Sai Kumar G, Sharma GT. 2012. Zygote: 1-11.
2. Mathieu ME, Saucourt C, Mournetas V, et al. 2012. Stem Cell Rev, 8: 1-15.
3. Thomson AJ, Pierart H, Meek S, et al. 2012. Cell Reprogram, 14: 112-22.
4. Li HandGrumet M. 2007. Glia, 55: 24-35.