Rabbit anti-β-Tubulin Polyclonal Antibody
货号-规格 | 货期 | 价格 | 数量 |
abs120147-50uL | 现货 | ¥800.00 | - + |
abs120147-100uL | 现货 | ¥1200.00 | - + |
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Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- Beta Tubulin (abs120147) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD | ||
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Beta Tubulin (abs120147) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD | ||
Blank control (blue line): Hela Primary antibody: Rabbit Anti-Beta Tubulin antibody Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. | ||
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